Sperm membrane integrity and thereby, sperm vitality is an important aspect to be assessed. It is especially in samples with severely compromised sperm parameters like membrane integrity which affects the fertilization potential of sperm and hence the assessment of sperm vitality is of great importance. The Wondersperm Vitality Kit, with its combined Eosin-Nigrosin solution allows rapid assessment of sperm viability.
The intact plasma membrane of the viable spermatozoa will not allow the stain to permeate and thus, viable spermatozoa will appear colourless, as opposed to the non viable spermatozoa with compromised membrane integrity, which will appear pink. Estimation of vitality is a simple calculation of the ratio of live and dead sperm using brightfield microscopy.